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Known worldwide as the standard introductory text to this important and exciting area of study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within.

 

Gene Cloning and DNA Analysis: An Introduction, 8th Edition contains updated and extended coverage of gene editing strategies like CRISPR/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-color illustrations are included to bring to life the comprehensive content. The book also covers topics like:

* The strategies used by researchers and industry practitioners to assemble genome sequences

* Next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes

* Includes the use and application of gene editing strategies

* Interbreeding between Neanderthals and Homo Sapiens

 

Gene Cloning and DNA Analysis: An Introduction, 8th Edition is an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis.

 

 

Contents:

Preface to the Eighth Edition xv Part I The Basic Principles of Gene Cloning and DNA Analysis 1 Why Gene Clorling and DNA Analysis are Important 3 Vectors for ~ede Cloning: Plasmids and Bacteriophages 15 Purification of DNA from Living Cells 29 Manipulation of Pwrfied DNA 53 Introduction of DNA into Living Cells 83 Clanihg Vectors for E. col; 101 Cloning Vectors for Eukaryotes 121 How to Obtain a Clone of a Sbecific Gene 145 The Polymerase Chain Reaction 169 Part I1 The Applications of Gene Clonhrg and DNA Analysis in Research is7 10 Sequencing Genes and Genomes 189 11 Studying Gene Expression and Function 217 12 Studying Genornes 243 13 Studying Transcriptomes and Proteomes 259 Part Ill The Applications of Gene Cloning and DNA Analysis in Biotechnology 27s 14 Production of Protein from Cloned Genes 277 15 Gene Cloning and DNA Analysis in Medicine 301 16 Gene Cloning and DNA Analysis in Agriculture 327 17 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 355 Glossary 377 Index 395