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Introduction to Biosensors

from Electric Circuits to immunosensors
Verfasser*in: Suche nach Verfasser*in Yoon, Jeong-Yeol
Verfasser*innenangabe: Jeong-Yeol Yoon
Jahr: 2013
Verlag: New York [u.a.], Springer
Mediengruppe: Buch
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Vorbestellen Zweigstelle: 07., Urban-Loritz-Pl. 2a Standorte: NN.BC FS.E Yoon / College 6a - Englisch Status: Verfügbar Frist: Vorbestellungen: 0

Inhalt

Verlagstext:Introduction to Biosensors: From Electric Circuits to Immunosensors discusses underlying circuitry of sensors for biomedical and biological engineers as well as biomedical sensing modalities for electrical engineers while providing an applications-based approach to the study of biosensors with over 13 extensive, hands-on labs. The material is presented using a building-block approach, beginning with the fundamentals of sensor design and temperature sensors and ending with more complicated biosensors. This book also: Provides electrical engineers with the specific knowledge they need to understand biological sensing modalities Provides biomedical engineers with a solid background in circuits and systems Includes complete coverage of temperature sensors, electrochemical sensors, DNA and immunosensors, piezoelectric sensors and immunosensing in a micofluidic device Introduction to Biosensors: From Electric Circuits to Immunosensors aims to provide an interdisciplinary approach to biosensors that will be appreciated by biomedical, biological, and electrical engineers. / AUS DEM INHALT: / / / Contents 1 Introduction. 1 1.1 Sensors 1 1.2 Transducers 2 1.3 Biosensors 5 1.4 Bioreceptors6 1.5 Transducers for Biosensors 12 1.6 Overview of This Textbook 13 Bibliography 15 2 Resistors 17 2.1 Electric Circuit 17 2.2 Current and Voltage 17 2.3 Resistance and Ohm's Law 19 2.4 Resistors in Series, or Voltage Divider 20 2.5 Potentiometer, or Pot 22 2.6 Resistors in Parallel, or Current Divider 23 2.7 Reading Resistor Values 24 2.8 Breadboards26 2.9 Laboratory Task 1: Resistors in Series27 2.10 Laboratory Task 2: Resistors in Parallel. 33 2.11 Laboratory Task 3: "Droop" 34 2.12 Laboratory Task 4: Potentiometer (Pot) 36 2.13 Further Study: Thévenin's Theorem 39 Bibliography 40 3 Diodes and Transistors. 41 3.1 Semiconductors 41 3.2 Diodes 42 3.3 Zener Diode45 3.4 Transistors 47 3.5 Operational Amplifier to Protect Your Circuit 52 3.6 Laboratory Task 1: LED 54 3.7 Laboratory Task 2: Zener Diode56 3.8 Laboratory Task 3: Transistor 58 Bibliography 61 4 Temperature Sensors 63 4.1 Thermocouple 63 4.2 Thermistor 65 4.3 Diode Temperature Sensor66 4.4 Transistor Temperature Sensor 68 4.5 Laboratory Task 1: Thermistor 69 4.6 Laboratory Task 2: Zener Diode Temperature Sensor 71 4.7 Laboratory Task 3: Transistor Temperature Sensor 74 Bibliography 78 5 Wheatstone Bridge79 5.1 Wheatstone Bridge 79 5.2 Strain Gauge 81 5.3 Cantilever Biosensor 82 5.4 Laboratory Task 1: Wheatstone Bridge 84 5.5 Laboratory Task 2: Wheatstone Bridge for a Thermistor 85 5.6 Laboratory Task 3: Wheatstone Bridge for a Strain Gauge88 Bibliography 90 6 Op-Amp 91 6.1 Op-Amp 91 6.2 Basics of Op-Amp 91 6.3 Voltage Follower or Buffer Op-Amp. 95 6.4 Non-Inverting Op-Amp 95 6.5 Inverting Op-Amp 97 6.6 Summing Op-Amp 98 6.7 Differential Op-Amp 98 6.8 Laboratory Task 1: Non-inverting Op-Amp Operation 99 6.9 Laboratory Task 2: Signal Conditioning for Temperature Sensor102 6.10 Further Study: Op-Amp Filters 106 Bibliography 108 7 Light Sensors. 109 7.1 Light. 109 7.2 Photoresistor 110 7.3 Photodiode 111 7.4 Phototransistor 115 7.5 Light-Emitting Diode (LED) 116 7.6 Laser Diode 118 7.7 Laboratory Task 1: Photoconductive Operation120 7.8 Laboratory Task 2: Photovoltaic Operation 123 Bibliography 126 8 Spectrophotometry127 8.1 Spectrophotometry 127 8.2 Spectrophotometry Biosensor Example: Pulse Oximeter131 8.3 Miniature Spectrophotometer 133 8.4 Optical Fibers 136 8.5 Laboratory Task 1: Hemoglobin Quantification with a Spectrophotometer 140 8.6 Laboratory Task 2: Hemoglobin Quantification with LED/PD Circuit 146 8.7 Laboratory Task 3: Meat Quality Monitoring with Reflection Probe 148 Bibliography 151 9 Fluorescence 153 9.1 Fluorescence 154 9.2 Fluorescent Dyes 156 9.3 Advanced Fluorescent Dyes: GFP, SYBR, and QD. 159 9.4 Autofluorescence 161 9.5 Detection of Fluorescence 163 9.6 Laboratory Task 1: 180° Back Scatter Fluorescence Detection for Fluorescein. 165 9.7 Laboratory Task 2: 90° Side Scatter Fluorescence Detection for Urine. 168 Bibliography 170 10 Electrochemical Sensors 171 10.1 Electrolytic and Electrochemical Cells 171 10.2 Ion-Selective Electrodes (ISEs; Potentiometric)176 10.3 pH Electrode (Potentiometric) 178 10.4 Amperometric Biosensors 179 10.5 Conductometric Biosensors 180 10.6 Laboratory Task 1: Buffer Preparations and Their pH Measurements 182 10.7 Laboratory Task 2: pH Meter Circuit 186 10.8 Laboratory Task 3: Fluoride Ion Selective Electrode with PH Meter 189 10.9 Laboratory Task 4: Fluoride Ion Selective Electrode with Circuit 191 Bibliography 192 11 Piezoelectric Sensors. 195 11.1 Piezoelectricity 195 11.2 Pressure Sensors. 198 11.3 Crystal Oscillators 198 11.4 Quartz Crystal Microbalance (QCM). 199 11.5 Viscoelasticity Consideration in QCM201 11.6 Flow Cell QCM as Biosensor 203 11.7 Laboratory Task 1: Quantifying BSA Adsorption on QCM Sensor 204 Bibliography 211 12 Glucose Sensors 213 12.1 Optical Glucose Sensor 213 12.2 Electrochemical Glucose Sensor 215 12.3 Other Electrochemical Biosensors 218 12.4 Continuous Glucose Monitoring (CGM) 219 12.5 Laboratory Task 1: Glucose Assay Kit with a Spectrophotometer 221 12.6 Laboratory Task 2: Glucose Assay Kit with LED/PD Circuit 224 12.7 Laboratory Task 3: Commercial Electrochemical Glucose Sensor 226 Bibliography 228 13 Immunosensors . 229 13.1 Enzyme-Linked Immunosorbent Assay (ELISA). 229 13.2 Antibodies 232 13.3 Antibody Fragments and Aptamers 234 13.4 Lateral-Flow Assay (LFA) 236 13.5 Optical Immunosensors 238 13.6 Surface Plasmon Resonance (SPR) Immunosensor 239 13.7 Electrochemical Immunosensors 241 13.8 Impedance Immunosensors: Interdigitated Microelectrode (IME) Immunosensor 243 13.9 Piezoelectric Immunosensors: QCM Immunosensor. 244 13.10 Immunosensing Kits Versus Handheld Immunosensors 245 13.11 Laboratory Task 1: Insulin ELISA Kit246 13.12 Laboratory Task 2: Insulin ELISA Kit with Smartphone Camera 248 13.13 Laboratory Task 3: Pregnancy Test (LFA) with Smartphone Camera. 251 13.14 Further Study: DNA Sensors 254 Bibliography 255 14 Lab-on-a-Chip Biosensors. 257 14.1 What Is Lab-on-a-Chip (LOC)? 257 14.2 How to Make LOCs: Photolithography and Soft Lithography 259 14.3 Early LOC: Capillary Electrophoresis (CE) 263 14.4 LOCs for Point-of-Care Testing (POCT) 265 14.5 Use of Optical Fibers in LOCs 267 14.6 Sample/Reagent Introduction 269 14.7 Mixing in LOC 270 14.8 Paper-Based LOCs 272 14.9 LOC Sensing with a Smartphone Camera 273 14.10 Other Applications of LOCs 274 14.11 Laboratory Task 1: Fabrication of LOC by Soft Lithography 275 14.12 Laboratory Task 2: Mixing in LOC 277 14.13 Laboratory Task 3: Fabrication of a Paper-Based LOC 280 14.14 Laboratory Task 4: Bradford Assay with Paper-Based LOC 284 14.15 Further Study: Latex Immunoagglutination Assay (LIA) in LOC287 14.16 Further Study: Polymerase Chain Reaction (PCR) in LOC 288 Bibliography 293 15 Nanobiosensors 299 15.1 Gold Nanoparticles (AuNPs) 299 15.2 Quantum Dots (QDs) 303 15.3 Zinc Oxide (ZnO) Nanostructures 305 15.4 Carbon Nanotubes (CNTs) and Graphene 307 15.5 Nanoporous Gold 310 15.6 Concluding Remarks310 Bibliography 311 Appendix: Microcontroller 313 Index 325

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Verfasser*in: Suche nach Verfasser*in Yoon, Jeong-Yeol
Verfasser*innenangabe: Jeong-Yeol Yoon
Jahr: 2013
Verlag: New York [u.a.], Springer
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Systematik: Suche nach dieser Systematik NN.BC, FS.E
Suche nach diesem Interessenskreis
ISBN: 978-1-4419-6021-4
2. ISBN: 978-1-4419-6022-1
Beschreibung: XIII, 262 S. : zahlr. Ill., graph. Darst.
Schlagwörter: Biosensor, Biochemischer Sensor
Suche nach dieser Beteiligten Person
Sprache: Englisch
Mediengruppe: Buch